Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.
نویسندگان
چکیده
In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 69 3 شماره
صفحات -
تاریخ انتشار 2003